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- Fred as RJ
- A Police Officer as Verne
- Scooby Doo as Hammy
- Batman as Ozzy
- Velma as Heather
- Robin as Lou
- Shaggy, Scrappy, and Scooby Dum as Bucky, Quillo, and Spike
- Daphne as Stella
- Ben Ravencroft as The Exterminator
- Phantom Virus as Vincent
- Lena as Glayds