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Shaggy as Mr. Conductor (Ringo Starr)

Scooby Doo as Mr. Conductor (George Carlin)

Daphne as Stacy Jones

Robin as Schemer

Scrappy Doo as Matt

Velma as Tanya

Scooby Dum as Dan

Sybella as Kara

Crystal as Becky

Fred as Harry Cupper

Batman as Billy Twofeathers

The Mayor of Oakhaven as JB King

Ben Ravencroft as Mr. Conductor's Evil Twin

Bill as Buster King

Mr. Beamon as Hobart Hume

Lena as Vickie

Uncle Nathaniel as Mr. Nicholas

The Theodore Tugboat Characters as The Thomas Characters

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