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- Matt as Scooby-Doo
- Mr. Counductor (Ringo Starr) as Shaggy
- Billy Twofears as Fred
- Stacey Jones as Velma
- Schemer as Scrappy Doo
- Mr. Counductor's Evil Twin as Villian
- The Evil Workers as Fake MP 1 and Fake MP 2
- Becky as Daphne