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- Thomas as Tubby the Tuba
- Percy as Pee-Wee the Piccolo
- James as The Herald
- Mr. Conductor (RS) as The Frog
- Gordon as The Great Pepperino
- Stacy Jones as Celeste
- DiDi as The Haughty Violin
- Rosie (from TTTE) as Mrs. Elephant
- Daisy as Mrs. Squeek
- Narrator as Himself