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- Barney (from Barney and Friends) as Mickey Mouse
- Becky as Minnie Mouse
- Stacy Jones as Daisy Duck
- BJ (from Barney and Friends) as Donald Duck
- Knuckles (from Sonic) as Goofy
- Matt (from Shining Time Station) as Pluto
- Mr. Conductor's Evil Twin as Pete
- Bulk and Skull (from Power Rangers) as Chip and Dale
- Carlos (from Barney and Friends) as Scrooge McDuck