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- Shrek as Mario
- Donkey as Luigi
- Gingy as Yoshi
- Lord Farquaad as Bowser
- Puss in Boots as Toad
- Princess Fiona as Princess Peach
- Queen Lilian as Princess Daisy
- Prince Charming as Bowser Jr.
- The Fairy Godmother as Wendy O. Koopa
- Rumplestiltskin as Kamek
- Pinochio as Donkey Kong
- The Big Bad Wolf as Diddy Kong
- Snow White as Birdo
- Lord Farquaad's henchmen as Goombas and Koopa Troopas