• Trevor as Gru
  • Nigel (Rio) as Vector
  • Eric Needles as Tim the Minion
  • Vana Glama as Margo
  • Candace (Phineas & Ferb) as Edith
  • Kitty Ko as Agnes
  • Grover (Sesame Street) as Dr. Nefario
  • Alameda Slim (Home on the Range) as Mr. Perkins

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