- Miles Monroe as Mario
- Luna Schlossesr as Princess Daisy
- Erno Windt as Luigi
- Dr. Nero as Donkey Kong
- Ellen Pogrebin as Princess Peach
- Dr. Melik as Diddy Kong
- Dr. Tyson as Bowser
- Dr. Dean as Bowser Jr.
- Dr. Aragon as Toad
- Dr. Orva as Princess Rosalina
- Rainer Krebs as Wario
- Herald Cohen as Yoshi
- Robot Tailor as Waluigi (uncredited)
- Evil Computer as Larry Koopa (uncredited)
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