• Miles Monroe as Mario
  • Luna Schlossesr as Princess Daisy
  • Erno Windt as Luigi
  • Dr. Nero as Donkey Kong
  • Ellen Pogrebin as Princess Peach
  • Dr. Melik as Diddy Kong
  • Dr. Tyson as Bowser
  • Dr. Dean as Bowser Jr.
  • Dr. Aragon as Toad
  • Dr. Orva as Princess Rosalina
  • Rainer Krebs as Wario
  • Herald Cohen as Yoshi
  • Robot Tailor as Waluigi (uncredited)
  • Evil Computer as Larry Koopa (uncredited)

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