• Michael Shayne as Mairo
  • Kay Bentley as Princess Peach
  • Helen Carlson as Princess Daisy
  • Everett Jason as Yoshi
  • George Trautwein as Diddy Kong
  • Carl Izzard as Mighty Plumber
  • Leander Jones - Porter as Donkey Kong
  • Tom Linscott as Wario
  • Engineer McGowan as Toadsworth
  • Conductor Lyons as Troff
  • Conductor Meyers as Scoff
  • Farm Lady as Princess Rosalina

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