- Jago as Mario
- TJ Combo as Luigi
- Orchid as Peach
- Audrey Charlotte (from The Joyful Masterpiece) as Daisy
- Snoopy as Yoshi
- Belle as Birdo
- Chief Thunder as Toad
- Bright River as Toadette
- Sabrewulf as Donkey Kong
- Tusk as Diddy Kong
- Glacius as Wario
- Cinder as Waluigi
- Tommy Pickles (from Rugrats) as Baby Mario
- Caillou (from Caillou) as Baby Luigi
- Max Goof (from Disney) as Koopa Troopa
- Bobby (from Disney) as ParaTroopa
- Fulgore as Bowser
- Timmy McNutly (from Rugrats) as Bowser Jr.
- Eyedol as Petey Piranha
- Gargos as King Boo
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