- Jago as Mario
- TJ Combo as Luigi
- Snoopy as Yoshi
- Orchid as Princess Toadstool
- Fulgore as Bowser
- Thunder as Oogtar
- Spinal as Larry "Cheasty" Koopa
- Cinder as Lemmy "Hip" Koopa
- Riptor as Morton "Big Mouth" Koopa
- Pinky (from Animaniacs) as Iggy "Hop" Koopa
- Brain (from Animaniacs) as Roy "Bully" Koopa
- Sadira as Wendy "Kootie Pie" O. Koopa
- Eyedol as Ludwig "Kookie" Von Koopa
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