• Sonic (from Sonic the Hedgehog) as Mr. Conductor #1
  • Toulouse (from The Aristocats) as Mr. Conductor #2
  • Amy Rose (from Sonic the Hedgehog) as Stacy Jones
  • Donald Duck (from Mickey Mouse) as Schemer
  • Hercules (from Hercules) as Harry Cupper
  • Baloo (from The Jungle Book) as Billy Twofeathers
  • Slightly the Lost Boy (from Peter Pan) as Matt
  • Marie (from The Aristocats) as Tanya
  • Mowgli (from The Jungle Book) as Dan
  • Daisy Duck (from Mickey Mouse) as Kara
  • Nala (from The Lion King) as Becky
  • Winnie the Pooh (from Winnie the Pooh) as Tex
  • Tigger (from Winnie the Pooh) as Rex
  • Piglet (from Winnie the Pooh) as Tito
  • Kessie (from Winnie the Pooh) as Didi
  • Kanga (from Winnie the Pooh) as Grace
  • Gaston (from Beauty and the Beast) as Buster the Bully
  • Scar (from The Lion King) as Mr. Conductor's Evil Twin
  • King Triton (from The Little Mermaid) as J.B. King
  • The Thomas & Friends characters as themselves

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