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Based on the movie Osmosis Jones.
- Sonic as Osmosis Jones
- Sally as Leah Estrogen
- Vector as Drix
- Eggman as Thrax
- Scratch, Grounder, Sleet, Dingo, Snively, Bocoe and Decoe as Other Germs
- Coconuts as Egg Germ
- Badniks as Egg Germs
- Tails as Mayor
- Knuckles as Tom Colonic
- Manic, Sonia, Shadow and random 100000000000000 hedgehogs as Police Cops
- Uncle Chuck as Chief of Police
- Jules as Sailor 1
- Locke as Sailor 2
- a random gremlin character from Sonic X as Scare Germ
- Frank as himself
- Shade (Osmosis Jones) as herself
- Ms. Boyd as herself
- Bob as himself
- Queen Aleena and a random hedgehog character as News Reporters
- Silver as Fire Chief
- a random echidna characters as Fire Guards
- Muttski as Fire Chief Dog
- Antoine as Nose Guard
- Cream as Boy
- Chao as Sneezes
- a random gremlin characters from Sonic X as Little Germ
No flames or spams.