- Emile de Becque as May
- Ensign Forbush as Misty
- Lieutenant Joseph Cable as Ash Ketchum
- Luther Bills as Pikachu
- Bloody Mary as Iris
- Liat as Nurse Joy
- Captain George Brackett as Professor Oak
- The Professor as Max
- Stewpot as Cilan
- Commander Bill Harbison as Tracey
- Lieutenant Buzz Adams as Meowth
- Henry as James
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