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- Fanboy as Scooby Doo
- Numbuh 2 as Shaggy
- Numbuh 5 as Velma
- Stan Marsh as Fred
- Wendy Testaburger as Daphne (Wendy Testaburger and Daphne are both Voiced By Mary Kay Bergman)
- Henrietta Von Marzipan as Crystal (Henrietta Von Marzipan and Crystal are both Voiced By Candi Milo)
- Yo as Amber
- Sigmund The Sorcerer as Lester (Sigmund The Sorcerer and Lester are both Voiced By Jeff Glen Bennett)
- Numbuh 86 as Dottie (Numbuh 86 and Dottie are both Voiced By Jennifer Hale)
- Chad Dickson as Steve
- Cree Lincoln as Laura
- Boog as Max