- Stan Marsh As Mario
- Kyle Broflovski as Luigi
- Eric Cartman as Bowser
- Wendy Testaburger as Princess Peach
- Kenny McCormick as Toad
- Baikinman (From Anpanman) as Iggy 'Hop' Koopa
- Horrorman (From Anpanman) as Morton 'Big Mouth' Koopa Jr.
- Dokichan (from Anpanman) as Wendy 'Kootie Pie' Koopa
- Snivley (From Sonic) as Dry Bowser
- Broly (From Dragon Ball) as Ludwig 'Kooky' Koopa
- Sephiroth (From Kingdom Hearts) as Roy 'Bully' Koopa
- Koopa & Goomba (From Mario) as Bowser's Minions
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