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- Numbuh 4 as Scooby Doo
- Numbuh 2 as Shaggy
- Numbuh 5 as Velma
- Stan Marsh as Fred
- Wendy Testaburger as Daphne (Wendy Testaburger and Daphne are both Voiced By Mary Kay Bergman)
- Mushi Sanban as Lena (Mushi Sanban and Lena are both Voiced By Tara Strong)
- Cree Lincoln as Simone