• Jenny (XJ9) (from My Life as a Teenage Robot) as Dory
  • Spongebob as Marlin
  • Patrick as Nemo
  • Mrs. Puff as Jenny
  • Mr. Krabs as Charlie
  • Homestar Runner (from Homestar Runner) as Bailey
  • Mindy as Destiny
  • Strong Bad (from Homestar Runner) as Hank
  • Joe (from Family Guy) as Gill
  • Agnes Skinner (from The Simpsons) as Deb
  • Plankton as Fluke
  • Superintendent Chamlers (from The Simpsons) as Rudder
  • Otto (from The Simpsons) as Mr. Ray
  • Spongebob's Dad as The School of Moonfish
  • Ned Flanders (from The Simpsons) as Jacques
  • Harold as Crush
  • Gary the Snail as Squirt
  • Sideshow Bob (from The Simpsons) as Becky