- Dr. Sam Foster as Mario
- Lila Culpepper as Princess Peach
- Henry Letham as Yoshi
- Dr. Leon Patterson as Diddy Kong
- Dr. Beth Levy as Birdo
- Athena as Princess Daisy
- Dr. Bradley Ren as Luigi
- Maureen Letham as Pauline
- Toni as Princess Rosalina
- The Professor as Toadsworth
- The Book Store Owner as Wario
- Officer #1 as Donkey Kong
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