• Dr. Sam Foster as Mario
  • Lila Culpepper as Princess Peach
  • Henry Letham as Yoshi
  • Dr. Leon Patterson as Diddy Kong
  • Dr. Beth Levy as Birdo
  • Athena as Princess Daisy
  • Dr. Bradley Ren as Luigi
  • Maureen Letham as Pauline
  • Toni as Princess Rosalina
  • The Professor as Toadsworth
  • The Book Store Owner as Wario
  • Officer #1 as Donkey Kong

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