- Luke as Tony Stark/Iron Man
- Haku (from The Spirit Away) as Steve Rogers/Captain America
- Chief Bogo as Dr. Bruce Banner
- Mayor Lionheart as The voice of Hulk
- Sean (from Journey 2: The Mysterious Island) as Thor
- Lin (from The Spirit Away) as Natasha Romanoff/Black Widow
- Yax as Clint Barton/Hawkeye
- Junior and Luke's Owner as Maria Hill
- Amos Slade (from The Fox and the Hound) as Sam Wilson/Falcon
- Benjamin Clawhauser as Nick Fury
- Hans (from Frozen) as Ultron
- Bellwether as Wanda Maximoff/Scarlet Witch
- Mr. O'Hare (from Dr. Seuss' The Lorax) as James Rhodes/War Machine
- Tommy (from Arthur) as Baron Strucker
- Mel (from The Secret Life of Pets) as Vision
- Tessa Yeager (from Transformers: Age of Extintion) as Peggy Carter
- Loco (from A Bug's Life) as Heimdall
- Grammy Norma (from Dr. Seuss' The Lorax) as Laura Barton
- Timmy (from Arthur) as Thanos
- Axel (from A Bug's Life) as Erik Selvig
- Junior as World War II Veteran
- Snowball as Ulysses Klaw
- Baymax (from Big Hero 6) as Dr. Helen Cho
- Ramone (from Cars) as Doctor List
- Chloe as Madame B.
- Young Tod (from The Fox and the Hound) as F.R.I.D.A.Y.
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