- Babydoll as Princess Daisy
- Sweet Pea as Princess Peach
- Rocket as Princess Rosalina
- Blondie as Toadette
- Amber as Birdo
- Madame Vera Gorski / Dr. Vera Gorski as Toadiko
- Blue Jones as Mario
- The Doctor / The High Roller as Luigi
- The Wise Man / The General / The Bus Driver as Toad
- The Stepfather as Yoshi
- Earl as Donkey Kong
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