- Jane Falbury as Princess Peach
- Joe D. Ross as Mario
- Orville Wingait as Yoshi
- Abigail Falbury as Birdo
- Esme as Princess Daisy
- Herb Blake as Luigi
- Jasper G. Wingait as Donkey Kong
- Sarah Higgins as Pauline
- Artie as Wario
- Harrison I. Keath as Diddy Kong
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