- Joseph C. "Joe" Gills as Mario
- Norma Desmond as Princess Daisy
- Maximillian "Max" von Mayerling as Toad
- Betty Schaefer as Pauline
- Sheldrake as Luigi
- Morino as Donkey Kong
- Arthur "Artie" Green as Yoshi
- Undertalker as Diddy Kong
- Finance Man #1 as Wario
- Finance Man #2 as Waluigi
- Cecil B. DeMille as Toadsworth
- Hedda Hopper as Princess Peach
- and more
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