- Sunshine (fron TUGS) as Milo
- Mario (from Super Mario Bros) as Oscar
- Emily (from Theodore Tugboat) as Bea
- Pudge (from Cats Don't Dance) as Albert Glass
- Inez (from Cyberchase) as Clamantha
- Marge Simpson (from The Simpsons) as Shellsea
- Blubber Bear (from Wacky Races) as Mr. Baldwin
- Zorran (from TUGS) as Jocktopus
- Buster (from Arthur) Coach Salmons
- Pooh (fromm Winnie the Pooh) as Jumbo Shrimp
- Captian Star (from TUGS) as Mr. Mussels
- Laura (from VeggieTales) as Finberley
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