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- Ten Cents (from TUGS) as Spongebob
- Thomas (from TTTE) as Patrick
- Marlin (from Finding Nemo) as Squidward
- Lillie Lightship (from TUGS) as Sandy
- Big Bird (from Sesame Street) as Mr. Krabs
- Pingu (from Pingu) as Gary
- Rosie (from TTTE) as Pearl
- Little Miss Sunshine (The Mr. Men Show) as Mrs. Puff
- Captain Zero and the Z-Stacks (from TUGS) as Plankton
- Robot Jones (from Whatever Happened to Robot Jones?) as Larry
- Diesel 10 (from Thomas and the Magic Railroad) as The Flying Dutchman