- Donald Duck (from Mickey Mouse) as RS Mr. Conductor
- Gus Goose (from Mickey Mouse) as GC Mr. Conductor
- Daisy Duck (from Mickey Mouse) as Stacy Jones
- Daffy Duck (from Looney Tunes) as Schemer
- Rafiki (from The Lion King) as Harry Cupper
- Sir Ector (from The Sword In The Stone) as Billy Twofeathers
- Billy Shoepack (from TUGS) as Matt
- Babs Bunny (from Tiny Toon Adventures) as Tanya
- Baloo (from The Jungle Book) as Dan
- Snow White (from Snow White) as Kara
- Sis (from Robin Hood) as Becky
- Sykes (from Oliver & Company) as Mr. Conductor's Evil Twin
- The Phineas & Ferb Characters as The Thomas Characters
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