• Trixie Lee as Princess Peach
  • Gerry Lester as Mario
  • Lois Allen as Princess Daisy
  • Babe Dugan as Birdo
  • Bud as Yoshi
  • Albama as Toadette
  • Lt. Dick "Tex" Prince as Toad
  • Chick as Wario
  • Sunny as Princess Rosalina
  • Speed Allen as Oogtar
  • Al Moore as Donkey Kong
  • T. P. Lester as Diddy Kong

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