- Pierre Dulaine as Mickey Mouse
- Principal Augustine James as Clarabelle Cow
- Mr. Joseph Temple as Professor Ludwig Von Drake
- Jason Rockwell as Donald Duck
- LaRhette Dudley as Ortensia
- Ramos as Pluto
- Danjou as Goofy
- Sasha as Minnie Mouse
- Monster as Huey Duck
- Caitlin as Daisy Duck
- Eddie as Horace Horsecollar
- Kurd as Louie Duck
- Trey as Scrooge McDuck
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