- Ivan Dragomiloff as Mario
- Sonya Winter as Princess Peach
- Lord Bostwick as Toad
- General von Pinck as Toadsworth
- Monsieur Lucoville as Yoshi
- Herr Weiss as Luigi
- Madame Otero as Princess Rosalina
- Cesare Spado as Wario
- Monsieur Popescu as Donkey Kong
- Baron Muntzof as Oogtar
- Eleanora Spado as Princess Daisy
- Angelo as Detective Herlock Solmes
- Swiss Peasant as Diddy Kong
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