- Lucy Warriner as Princess Peach
- Jerry Warriner as Mario
- Dan Leeson as Yoshi
- Armand Duvalle as Luigi
- Aunt Patsy as Princess Daisy
- Barbara Vance as Birdo
- Mrs. Leeson as Baby Princess Daisy
- Dixie Belle Lee as Princess Rosalina
- Frank Randall as Diddy Kong
- Mr. Vance as Donkey Kong
- Mrs. Vance as Toadette
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