- Josh Barkley as Mario
- Dinah Barkley as Princess Rosalina
- Ezra Miller as Luigi
- Mrs. Livingston Belney as Princess Peach
- Shirlene May as Princess Daisy
- Jacques Pierre Barredout as Toad
- Bert Feisher as Yoshi
- Pamela Driscoll as Birdo
- The Judge as Bowser
- Ladislaus Ladi as Donkey Kong (uncredited)
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