- Jason Bourne as The Cat in the Hat
- Marie Helena Kreutz as Daisy-Head Mayzie
- Alexander Conklin as Thing One
- Ward Abbott as Thing Two
- The Professor as The Grinch
- Nykwana Wombosi as Horton the Elephant
- Danny Zorn as Yertle the Turtle
- Nicolette Parsons as Cindy Lou Who
- Research Tech #1 as Joey
- Research Tech #2 as Mr. Knox
- Giancarlo as Norval the Fish
- Eamon as Mack the Turtle
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