- Jason Bourne as Tramp
- Marie Helena Kreutz as Lady
- Alexander Conklin as Tony
- Ward Abbott as Scamp
- The Professor as Buster
- Nykwana Wombosi as Jim Dear
- Danny Zorn as Mooch
- Nicolette Parsons as Scamp's Sister
- Research Tech #1 as Jock
- Research Tech #2 as The Beaver
- Giancarlo as The Dog Catcher
- Eamon as Scratchy
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