- Jason Bourne as Buster Bunny
- Marie Helena Kreutz as Mary Melody
- Alexander Conklin as Plucky Duck
- Ward Abbott as Dizzy Devil
- The Professor as Montana Max
- Nykwana Wombosi as Calamity Coyote
- Danny Zorn as Hamton J. Pig
- Nicolette Parsons as Babs Bunny
- Research Tech #1 as Gogo Dodo
- Research Tech #2 as Li'l Sneezer
- Giancarlo as Bookworm
- Eamon as Little Beeper
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