- Kenneth James Kitsom as Darkwing Duck
- Dr. Marta Shearing as Morgana
- Eric Buyer as Launchpad McQuack
- CIA Deputy Director Pamela Landy as Liquidator
- Terrence Ward as Bushroot
- Mark Turso as Stegmutt
- Dr. Donald Foite as Gizmoduck
- LARX-03 as J. Gander Hooter
- and more
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