- Kenneth James Kitsom as Diddy Kong
- Dr. Marta Shearing as Dixie Kong
- Eric Buyer as Donkey Kong
- CIA Deputy Director Pamela Landy as Candy Kong
- Terrence Ward as King K. Rool
- Mark Turso as Cranky Kong
- Dr. Donald Foite as Bluster Kong
- LARX-03 as Krusha
- and more
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