• Kenneth James Kitsom as Mickey Mouse
  • Dr. Marta Shearing as Minnie Mouse
  • Eric Buyer as Goofy
  • Mark Turso as Pluto
  • Terrence Ward as Figaro
  • Outcome 3 as Willy the Giant
  • CIA Deputy Director Pamela Landy as Daisy Duck
  • Dr. Albert Hirsch as Donald Duck
  • Noah Vosen as Max Goof
  • CIA Director Ezra Kramer as Chip
  • Dita as Clarabelle Cow
  • Dr. Donald Foite as Dale
  • Vendel as Jiminy Cricket
  • Factory Owner as Casey Junior
  • Dr. Connie Dowd as Clara Cluck
  • LARX-03 as Huey Duck
  • Ray Wills as Dewey Duck
  • Joseph as Louie Duck
  • The Captain as Scrooge McDuck
  • Simon Ross as Horace Horsecollar (archive footage)

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