- Kenneth James Kitsom as Basil
- Dr. Marta Shearing as Olivia Flaversham
- Eric Buyer as Ratigan
- CIA Deputy Director Pamela Landy as The Singing Female Mouse
- Terrence Ward as Toby
- Mark Turso as Hiram Flaversham
- Dr. Donald Foite as Fidget
- LARX-03 as Dawson
- and more
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