- Jason Bourne as Mickey Mouse
- Nicolette "Nicky" Parsons as Daisy Duck
- Noah Vosen as Professor Ludwig Von Drake
- Ezra Kramer as Donald Duck
- Simon as Goofy
- Paz as Pluto
- Dr. Albert Hirsch as Hroace Horsecollar
- Pamela "Pam" Landy as Minnie Mouse
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