- Gru (from Despicable Me) as Milo Thatch
- Lucy Wilde (from Despicable Me 2) as Princess Kida
- Anna (from Frozen) as Audrey Rocio Ramirez
- Oaken (from Frozen) as Mr. Harcourt
- King Harold (from The Simpsons) as Dr. Sweet
- Eggs as Mole
- Young Once-ler (from Alvin and the Chipmunks) as Vinny
- Tulio (from Rio) as Preston Whitmore
- Dr. Nefario (from Despicable Me) as Cookie
- Miss Finster (from Recess) as Wilhelmina Packard
- Mother Gothel (from Tangled) as Helga Sinclair
- Archibald Snatcher as Lyle T. Rourke
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