- Gru (from Despicable Me) as Dave Douglas
- Lucy Wilde (from Despicable Me 2) Rebeeca Douglas
- Winnie as Carly Douglas
- Eggs as Josh Douglas
- Joe the Lion (from Zookeeper) as Ken Hollister
- Archibald Snatcher as Dr. Kozak
- Sulley (from Monsters, Inc. and University) as Justin Forrester
- The Lorax (from The Lorax) as Trey
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