- Lieutenant Commander Philip Francis Queeg as Mario
- Lieutenant Barney Greenwald as Yoshi
- Lieutenant Steve Marky as Luigi
- Lieutenant Tom Keefer as Chunky Kong
- Ensign Willis Seward "Willie" Keith as Mallow
- May Wynn as Princess Peach
- Lieutenant Commander De Vriess as Toad
- Seaman as Wario
- Lieutenant commander Challee as Mighty Plumber
- Mrs. Keith as Princess Daisy
- Chief Budge as Donkey Kong
- Ensign Barney Harding as Diddy Kong
- and more
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