- Lieutenant Commander Philip Francis Queeg as Mickey Mouse
- Lieutenant Barney Greenwald as Donald Duck
- Lieutenant Steve Marky as Scrooge McDuck
- Lieutenant Tom Keefer as Horace Horsecollar
- Ensign Willis Seward "Willie" Keith as Scrooge McDuck
- May Wynn as Minnie Mouse
- Lieutenant Commander De Vriess as Dewey Duck
- Seaman as Louie Duck
- Lieutenant commander Challee as Huey Duck
- Mrs. Keith as Clara Cluck
- Chief Budge as Goofy
- and more
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