- Sarah Bailey as Princess Peach
- Nancy Downs as Wendy O. Koopa
- Bonnie Hyper as Princess Daisy
- Rochelle Zimmerman as Toadette
- Chris Hooker as Bowser
- Mr. Bailey as Luigi
- Laura Lizzie as Birdo
- Mitt as Mario
- Trey as Yoshi
- Lirio as Princess Rosalina
- Street Preacher as Donkey Kong
- Doctor as Toadsworth
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