- Batman/Bruce Wayne as Mario
- The Joker as Bowser
- Alfred as Luigi
- Rachel Dawes as Princess Rosalina
- Gordon's Wife as Princess Peach
- Gordon's Daughter as Princess Daisy
- Gordon's Son as Yoshi
- Lucius Fox as Toad
- Lau as Waluigi
- Harvey Dent/Two Face as Wario
- Mayor as Donkey Kong
- Commissioner Gordon as Diddy Kong
- and more
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