The Disney School Bus is a Disney spoof to The Magic School Bus, complete with clips from various Disney shows.
- Julie Kane (from The Adventures of Julie Kane) as Miss Valerie Felicity Frizzle
- Winnie The Pooh (from The New Adventures of Winnie The Pooh) as Liz
- Todd Daring (from The Replacements) as Arnold Perlstein
- Randy Cunningham (from Randy Cunningham: 9th Grade Ninja) as Carlos Ramon
- Mitchie O'Hara (from Accidentally Adventures) as Phoebe Therese
- Penn Zero (from Penn Zero: Part Time Hero) as Tim Jamal
- Star Butterfly (from Star vs. The Forces of Evil) as Dorothy Ann
- Amethyst Giger (from Livin' The Life With The Stereotypes) as Wanda Li
- Kick Buttowski (from Kick Buttowski: Suburban Daredevil) as Ralphie Tennelli
- Mabel Pines (from Gravity Falls) as Keesha Franklin
- Julie Kane: Seat belts, everyone!
- Todd Daring: Please let this be a normal field trip.
- Amethyst: With the frizz?
- Kids: No way!
- Chorus: Cruisin' on down Main Street You're relaxed and feelin' good Next thing that you know you're seein' Octopus in the neighborhood! Surfin' on a sound wave Swingin' through the stars Take a left at your intestine Take your second right past Mars. On THE DISNEY SCHOOL BUS Navigate a nostril Climb on THE DISNEY SCHOOL BUS Spank a plankton, too On our Disney School Bus Raft a river of lava On THE DISNEY SCHOOL BUS Such a fine thing to do! So strap your bones right to the seat Come on in and don't be shy -- Just to make your day complete You might get baked into a pie On THE DISNEY SCHOOL BUS... Step inside -- it's a wilder ride! Come on -- ride on THE DISNEY SCHOOL BUS!