- Yansci "Jenny" Dolly as Agnes
- Harry Fox as Dave the Minion
- Rozsika "Rosie" Dolly as Margo
- Uncle "Latsie" as Mr. Perkins
- Tony, Duke of Breck as Paul the Minion
- Irving Netcher as Dr. Nefario
- Professor Winnup as Vetor
- Ignatz Tsimmis as Tim the Minion
- Lenora Baldwin as Edith
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