- Yansci "Jenny" Dolly as Lucy Wilde
- Harry Fox as Gru
- Rozsika "Rosie" Dolly as Margo
- Uncle "Latsie" as Dr. Nefario
- Tony, Duke of Breck as Dave the Minion
- Irving Netcher as Silas Ramsbottom
- Professor Winnup as El Macho
- Ignatz Tsimmis as Floyd Eagle-San
- Lenora Baldwin as Shannon
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