- Hazel Grace Lancaster as Princess Rosalina
- Augustus Waters as Mario
- Isaac as Luigi
- Frannie Lancaster as Princess Peach
- Michael Lancaster as Toad
- Patrick as Yoshi
- Lidewik Vligenthart as Princess Daisy
- Peter van Houten as Wario
- Mrs. Waters as Birdo
- Mr. Waters as Waluigi
- Dr. Maria as Toadette
- Monica as Wendy O. Koopa
- John Green as Oogtar (cameo)
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