• Hazel Grace Lancaster as Princess Rosalina
  • Augustus Waters as Mario
  • Isaac as Luigi
  • Frannie Lancaster as Princess Peach
  • Michael Lancaster as Toad
  • Patrick as Yoshi
  • Lidewik Vligenthart as Princess Daisy
  • Peter van Houten as Wario
  • Mrs. Waters as Birdo
  • Mr. Waters as Waluigi
  • Dr. Maria as Toadette
  • Monica as Wendy O. Koopa
  • John Green as Oogtar (cameo)

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