- Hazel Grace Lancaster as Annie Sue Pig
- Augustus Waters as Gonzo the Great
- Isaac as Kermit the Frog
- Frannie Lancaster as Miss Piggy
- Michael Lancaster as Fozzie Bear
- Patrick as Sweetums
- Lidewik Vligenthart as Camilla the Chicken
- Peter van Houten as Rizzo the Rat
- Mrs. Waters as Wanda
- Mr. Waters as Wayne
- Dr. Maria as Foo-Foo
- Monica as Janice
- John Green as Dr. Bunsen Honeydew (cameo)
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